Production of -amylase from Penicillium brevicompactum was investigated in solid-state fermentation (SSF) using as substrate wheat bran (WB), rye bran (RB) and barley bran (BB) enriched with different amount of glucose or not. Consumption of glucose by fungal cells in WB and RB cultures was more effective than BB cultures. Optimal moisture levels for maximal -amylase production in WB, RB and BB cultures without glucose were 55, 65 and 35 %, respectively. Water absorption capacities of substrates were WB>RB>BB. In SSF process, decrease in enzyme production was greater in high moisture level than optimal moisture level. According to the other two cultures, production of -amylase from P. brevicompactum was strongly inhibited in higher moisture levels than optimal moisture levels in BB cultures enriched with 500 mg/g glucose.
Bilal Balkan
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