Biological active diamines, cadaverine and putrescine together with 1,3-propylenediamine and 1,2-propylenediamine react with 2-hydroxynaphthaldehyde to form bis (2-hydroxynaphthaldehyde) 1,5-diiminopentane (HN(2)CA), bis(2-hydroxynaphthaldehyde) 1,4-diiminobutane (HN2Pu) bis (2-hydroxynaphthaldehyde) 1,2-diiminopropane (HN(2)Pn) and bis (2-hydroxynaphthaldehyde) 1,3-diiminopropane (HN2PR). The derivatives are observed to be fluorescent in visible region. The wavelength of excitation (Ex) and emission (Em) were determined for optimal fluorescence of derivatives. The linear calibration for each of the diamine is observed at 0.32-1.6 mu g/ml. The diamine derivatives HN2Pu, HN(2)Pn also separated on Nova Pak C-18 column when eluted with methanol. The spectrofluorimetric detection was obtained at 450 nm using Ex at 315 nm.


M B RIND ,M Y KHUHAWAR ,M A QURSHI ,